Well volume (in mL)   Typical value for 96well plate is 0.1 mL.

Starting dilution   Must be ≤ 1 (e.g. 10fold as 0.1, 4fold as 0.25).

Dilution factor   Must be < 1 (e.g. 10fold as 0.1, 4fold as 0.25).

# dilutions   Typically 7 or 8 or 11 or 12.

# repeats/dilution   Typically 4 or 6 or 8.
